Avian influenza H5N8 has been confirmed in a second backyard flock near Thornton, Lancashire, just a few days after an outbreak in a flock of 30 chickens.
This outbreak was detected in a flock of nine chickens and ducks on 6th May.
A 3km protection zone and a 10km surveillance zone has been put in place around the affected area to limit the risk of disease spreading further.
Some birds in the flock had died and the remainder have been humanely culled. A full investigation is underway to identify the source of this infection.
Defra confirmed another case of H5N8 in a backyard flock of 30 chickens earlier on 4th May, which was also in Thornton.
At the end of last month Defra had announced that the avian flu prevention zone and ban on poultry gatherings would be relaxed from 15 May - if there were no more H5N8 findings.
The prevention zone requires poultry keepers to follow certain measures to reduce the risk of infection from wild birds. This zone was introduced following outbreaks of H5N8 in poultry and wild birds in several countries across Europe to try and prevent the virus spreading further.